An increase in the number of peroxisomes is coupled to the initial infection stage and stress response of Botrytis cinerea

Abstract Peroxisomes are very important organelles in eukaryotic cells and participate various biological processes, including pathogen–host interactions. A variety of proteins involved peroxisome proliferation metabolic activity within peroxisomes have been shown to be essential for full virulence several phytopathogenic fungi. However, the effects changes number pathway on pathogenicity Botrytis cinerea rarely reported. In this study, by analysing transcriptome data RT-qPCR validation, we found that more than half genes annotated B. were upregulated twofold between mycelial samples cultured medium with tomato leaves without leaves. strain fluorescently labelled was obtained overexpression GFP fused peroxisomal targeting signal 1 (the tripeptide ‘SKL’). The addition liquid induced a significant increase peroxisomes, ?-oxidation level, H 2 O content, acetyl-CoA level mycelia. When oleic acid as sole carbon source, formation infection-related structures their penetration into plant associated activity. Furthermore, lipid metabolism increased response different extracellular stresses . Taken together, our results confirmed activation contributes initial infection ability cope environmental stress.